Application of color digital holographic microscopy for analysis of stained tissue sections

Xiaoli Mo; Björn Kemper; Patrik Langehanenberg; Angelika Vollmer; Jinghui Xie; Gert von Bally

doi:10.1117/12.831476

1 July 2009 Application of color digital holographic microscopy for analysis of stained tissue sections

Xiaoli Mo; Björn Kemper; Patrik Langehanenberg; Angelika Vollmer; Jinghui Xie; Gert von Bally

Author Affiliations +

Proceedings Volume 7367, Advanced Microscopy Techniques; 736718 (2009) https://doi.org/10.1117/12.831476
Event: European Conferences on Biomedical Optics, 2009, Munich, Germany

Abstract

The analysis of stained tissue sections represents an important tool in medical diagnostics. Color digital holographic microscopy offers subsequent multi-focus true color imaging with simultaneous quantitative phase contrast analysis. Investigations on digital recording and numerical reconstructions of color digital holographic images have been performed by applying a transmission microscope experimental setup in holographic off-axis geometry. Three monochromatic digital holograms with different wavelengths in red, green and blue spectral range are recorded. After digital holographic refocusing and compensation for aberrations of the microscope imaging system by digital image correlation the numerically reconstructed amplitude distributions are combined into color images. The applicability of the method is demonstrated by results obtained from stained intestine tissue sections.

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Xiaoli Mo, Xiaoli Mo, Björn Kemper, Björn Kemper, Patrik Langehanenberg, Patrik Langehanenberg, Angelika Vollmer, Angelika Vollmer, Jinghui Xie, Jinghui Xie, Gert von Bally, Gert von Bally, } "Application of color digital holographic microscopy for analysis of stained tissue sections", Proc. SPIE 7367, Advanced Microscopy Techniques, 736718 (1 July 2009); doi: 10.1117/12.831476; https://doi.org/10.1117/12.831476

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