Controlling the emission of organic dyes for high sensitivity and super-resolution microscopy

Thorben Cordes; Ingo H. Stein; Carsten Forthmann; Christian Steinhauer; Monika Walz; Wolfram Summerer; Britta Person; Jan Vogelsang; Philip Tinnefeld

doi:10.1117/12.831495

1 July 2009 Controlling the emission of organic dyes for high sensitivity and super-resolution microscopy

Thorben Cordes, Ingo H. Stein, Carsten Forthmann, Christian Steinhauer, Monika Walz, Wolfram Summerer, Britta Person, Jan Vogelsang, Philip Tinnefeld

Author Affiliations +

Proceedings Volume 7367, Advanced Microscopy Techniques; 73671D (2009) https://doi.org/10.1117/12.831495
Event: European Conferences on Biomedical Optics, 2009, Munich, Germany

Abstract

In this paper we show that the emission of ordinary organic dyes can be controlled in order to increase photostability or to induce long off-states for superresolution microscopy. We therefore extend a recently introduced concept that utilizes triplet-state quenching via redox-reactions and recovery of the electronic ground-state by complementary redoxreactions: it is shown that different reagents in an oxidizing and reducing system (ROXS) can positively influence the fluorescence properties of organic dyes. In more detail, the effects of Trolox, a ferrocene-based compound, an oxidized quinone derivative of Trolox and nitrobenzoic acid are investigated and compared to the prototypical compounds ascorbic acid and N,N methylviologen. While the redox potential is the most important parameter for the realization of the ROXS concept it is demonstrated that also kinetic aspects have to be taken into account to explain the properties of the specific redox agents. Photostabilization and the induction of off-states are of paramount importance for fluorescence microscopy in general and especially for superresolution microscopy based on "blinking" molecules.

Citation Download Citation

Thorben Cordes, Ingo H. Stein, Carsten Forthmann, Christian Steinhauer, Monika Walz, Wolfram Summerer, Britta Person, Jan Vogelsang, and Philip Tinnefeld "Controlling the emission of organic dyes for high sensitivity and super-resolution microscopy", Proc. SPIE 7367, Advanced Microscopy Techniques, 73671D (1 July 2009); https://doi.org/10.1117/12.831495

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available