MEMS-based confocal laser scanning microscope for in vivo imaging

Jürgen Helfmann; Rijk Schütz; Ingo Gersonde; Gerd Illing

doi:10.1117/12.831536

1 July 2009 MEMS-based confocal laser scanning microscope for in vivo imaging

Jürgen Helfmann, Rijk Schütz, Ingo Gersonde, Gerd Illing

Proceedings Volume 7367, Advanced Microscopy Techniques; 73671P (2009) https://doi.org/10.1117/12.831536
Event: European Conferences on Biomedical Optics, 2009, Munich, Germany

Abstract

With a cardanically mounted micromirror a confocal laser scanning microscope for in vivo imaging was built. A resolution of 0.6 μm laterally and 10 μm axially allows to image tissue and cells in good quality. Samples of skin and adhered cells are imaged either in reflection or in fluorescence with an excitation wavelength of 682 nm. Fluorescence of Indocyanine Green is detected in the wavelength range above 730 nm.

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Jürgen Helfmann, Rijk Schütz, Ingo Gersonde, and Gerd Illing "MEMS-based confocal laser scanning microscope for in vivo imaging", Proc. SPIE 7367, Advanced Microscopy Techniques, 73671P (1 July 2009); https://doi.org/10.1117/12.831536

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KEYWORDS

Microscopes

Confocal microscopy

Luminescence

In vivo imaging

Reflectivity

Skin

Laser scanners

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