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7 July 2009 Multiplexed diagnostics and spectroscopic ruler applications with terbium to quantum dots FRET
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Abstract
In this contribution we present the application of five different commercially available semiconductor core/shell quantum dots (Qdot® Nanocrystals - Invitrogen Corp.) as multiplexing FRET acceptors together with a commercial supramolecular terbium complex (Lumi4®-Tb - Lumiphore Inc.) as donor in a homogeneous immunoassay format. To realize the molecular recognition necessary for a FRET assay, the terbium complex was labeled to streptavidin (sAv- Lumi4-Tb) and the QDs were surface functionalized with biotin (Biot-QD). The biotin-streptavidin binding serves as a proof-of-principle representative for many biological interactions taking place on the nanometer scale (e.g. immunoassays). The presented FRET system can be efficiently used for the detection of inter- and intramolecular processes for clinical diagnostics and biomedical spectroscopy as well as molecular ruler applications and microscopy.
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Daniel Geissler, Nathaniel G. Butlin, Diana Hill, Hans-Gerd Löhmannsröben, and Niko Hildebrandt "Multiplexed diagnostics and spectroscopic ruler applications with terbium to quantum dots FRET", Proc. SPIE 7368, Clinical and Biomedical Spectroscopy, 73680P (7 July 2009); doi: 10.1117/12.831631; https://doi.org/10.1117/12.831631
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