Darkfield scattering spectroscopic microscopy evaluation using polystyrene beads

Michael Schmitz; René Michels; Alwin Kienle

doi:10.1117/12.831591

7 July 2009 Darkfield scattering spectroscopic microscopy evaluation using polystyrene beads

Michael Schmitz, René Michels, Alwin Kienle

Proceedings Volume 7368, Clinical and Biomedical Spectroscopy; 73681W (2009) https://doi.org/10.1117/12.831591
Event: European Conferences on Biomedical Optics, 2009, Munich, Germany

Abstract

Diameters of single polystyrene beads were determined within ~10 nm accuracy by comparing Mie theory oscillations and wavelength resolved measurements. The setup is realized with an axicon supported reflected darkfield microscope and is herein presented in detail. Further we explain a theoretical model considering the effective numerical aperature of the measuring system. A fitting algorithm allows rapid characterization of the sphere diameters.

Citation Download Citation

Michael Schmitz, René Michels, and Alwin Kienle "Darkfield scattering spectroscopic microscopy evaluation using polystyrene beads", Proc. SPIE 7368, Clinical and Biomedical Spectroscopy, 73681W (7 July 2009); https://doi.org/10.1117/12.831591

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