1 July 2009 Improving FRET detection in living cells
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Abstract
Unambiguous FRET detection in living cells is often challenging. Here we describe how the advantages of fluorescence lifetime sensing with FLIM, fluorophore selection, and critical environmental controls provide better FRET detection.
© (2009) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Ching-Wei Chang, Ching-Wei Chang, Mei Wu, Mei Wu, Sofia D. Merajver, Sofia D. Merajver, Mary-Ann Mycek, Mary-Ann Mycek, } "Improving FRET detection in living cells", Proc. SPIE 7370, Molecular Imaging II, 737007 (1 July 2009); doi: 10.1117/12.831718; https://doi.org/10.1117/12.831718
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