Time-resolved blood flow measurement in the in vivo mouse model by optical frequency domain imaging

Julia Walther; Gregor Mueller; Sven Meissner; Peter Cimalla; Hanno Homann; Henning Morawietz; Edmund Koch

doi:10.1117/12.831828

13 July 2009 Time-resolved blood flow measurement in the in vivo mouse model by optical frequency domain imaging

Julia Walther, Gregor Mueller, Sven Meissner, Peter Cimalla, Hanno Homann, Henning Morawietz, Edmund Koch

Proceedings Volume 7372, Optical Coherence Tomography and Coherence Techniques IV; 73720J (2009) https://doi.org/10.1117/12.831828
Event: European Conferences on Biomedical Optics, 2009, Munich, Germany

Abstract

In this study, we demonstrate that phase-resolved Doppler optical frequency domain imaging (OFDI) is very suitable to quantify the pulsatile blood flow within a vasodynamic measurement in the in vivo mouse model. For this, an OFDI-system with a read-out rate of 20 kHz and a center wavelength of 1320 nm has been used to image the time-resolved murine blood flow in 300 μμm vessels. Because OFDI is less sensitive to fringe washout due to axial sample motion, it is applied to analyze the blood flow velocities and the vascular dynamics in six-week-old C57BL/6 mice compared to one of the LDLR knockout strain kept under sedentary conditions or with access to voluntary wheel running. We have shown that the systolic as well as the diastolic phase of the pulsatile arterial blood flow can be well identified at each vasodynamic state. Furthermore, the changes of the flow velocities after vasoconstriction and -dilation were presented and interpreted in the entire physiological context. With this, the combined measurement of time-resolved blood flow and vessel diameter provides the basis to analyze the vascular function and its influence on the blood flow of small arteries of different mouse strains in response to different life styles.

Citation Download Citation

Julia Walther, Gregor Mueller, Sven Meissner, Peter Cimalla, Hanno Homann, Henning Morawietz, and Edmund Koch "Time-resolved blood flow measurement in the in vivo mouse model by optical frequency domain imaging", Proc. SPIE 7372, Optical Coherence Tomography and Coherence Techniques IV, 73720J (13 July 2009); https://doi.org/10.1117/12.831828

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