Determination of the optical properties of PNIPAAm gels used in biological applications

A. Singh; A. E. Karsten; I. Mputle; A. Chetty; Kersch Naidoo

doi:10.1117/12.831882

17 July 2009 Determination of the optical properties of PNIPAAm gels used in biological applications

A. Singh, A. E. Karsten, I. Mputle, A. Chetty, Kersch Naidoo

Proceedings Volume 7373, Therapeutic Laser Applications and Laser-Tissue Interactions IV; 737317 (2009) https://doi.org/10.1117/12.831882
Event: European Conferences on Biomedical Optics, 2009, Munich, Germany

Abstract

Poly(N-isopropylacrylamide) i.e. PNIPAAm is a temperature sensitive smart material which displays a lower critical solution temperature (LCST) at 33-35°C. At the lower critical solution temperature, the gel changes from hydrophilic to hydrophobic which has significant consequences in cell culturing. The first known measurements of the optical properties i.e. absorption (μ_a) and reduced scattering (μ'_s) coefficients, as a function of temperature, of a series of crosslinked PNIPAAm gels, using an Integrating Sphere setup, is presented at a wavelength of 632.8 nm. These properties showed a direct correlation between the scattering coefficient and the crosslinker density for the gels. The absorption properties correlated well with the known absorption characteristics of these gels.

Citation Download Citation

A. Singh, A. E. Karsten, I. Mputle, A. Chetty, and Kersch Naidoo "Determination of the optical properties of PNIPAAm gels used in biological applications", Proc. SPIE 7373, Therapeutic Laser Applications and Laser-Tissue Interactions IV, 737317 (17 July 2009); https://doi.org/10.1117/12.831882

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