17 July 2009 Evaluation of the PDT effect of Foscan and Fospeg in the LNCaP human prostate cancer cell line
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Abstract
In this paper the cytotoxic effect of m-THPC, Foscan®, as well as of the liposomal formulation of m-THPC, Fospeg®,(kind offer of Biolitec) were studied post PDT in the human prostate cancer cell line LNCaP. The cells were incubated for 24h with 0.15 μg/ml and 1.2 μg/ml Foscan® and Fospeg®. Irradiation was performed with a 652nm laser and energy doses 180, 360 and 540mJ/cm2. The effect was assessed by the MTT viability test 24h after irradiation. Also the intracellular localization of Foscan® and Fospeg® was monitored by using Laser Scanning Confocal Microscopy Imaging. The results showed no dark toxicity either with Foscan® or Fospeg® at any concentration. Also irradiation at each energy dose in the absence of any photosensitizer, did not affect cellular viability. The cellular death caused after Photodynamic Treatment was dependent on m-THPC concentration and formulation, as well as the delivered energy dose. Fospeg® was more effective as LD50 was achieved with 0.15μg/ml at 180mJ/cm2 while for the same cytotoxic result 1.2μg/ml Foscan® was needed. Images from confocal microscopy revealed higher fluorescence intensity in the cytoplasm after incubation with Fospeg®, than upon incubation with Foscan® under the same experimental conditions.
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A. Petri, A. Petri, M. Kyriazi, M. Kyriazi, E. Alexandratou, E. Alexandratou, M. Rallis, M. Rallis, S. Gräfe, S. Gräfe, D. Yova, D. Yova, } "Evaluation of the PDT effect of Foscan and Fospeg in the LNCaP human prostate cancer cell line", Proc. SPIE 7373, Therapeutic Laser Applications and Laser-Tissue Interactions IV, 73731I (17 July 2009); doi: 10.1117/12.831881; https://doi.org/10.1117/12.831881
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