24 November 2010 Interferometry for topographical diagnostics of RBCs in optical tweezers
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Proceedings Volume 7376, Laser Applications in Life Sciences; 73760G (2010) https://doi.org/10.1117/12.878959
Event: Laser Applications in Life Sciences 2010, 2010, Oulu, Finland
Abstract
Simultaneous non-invasive trapping and topography of erythrocytes by interferometric fringe projection profilometry has been presented. Sinusoidal fringes were generated with the help of compact Michelson interferometer (CMI) developed by coating a thin reflective layer (~100μm) of Al2O3 on one face of a cubic beam splitter. An external mirror was mounted on XYZ translational stage to control the fringe frequency and orientation. Red He-Ne laser was used to generate the sinusoidal probe -interferogram using CMI to project onto the green-laser (cw) trapped healthy and deceased RBCs separately. Information coded reflected interferograms exhibits characteristic fringe-deviation with respect to probe-pattern for both the healthy and defective RBCs. Fourier transform analysis was adopted to retrieve the phase-map which can be exploited for topography, size determination and refractive-index of RBC. Refractive-index change is directly related with hemoglobin concentration of RBCs at any specific physiological state and hence information about health status and disease progression can be anticipated.
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Ranjeet Kumar, Sarita Saraswati, Chandra Shakher, Dalip S. Mehta, "Interferometry for topographical diagnostics of RBCs in optical tweezers", Proc. SPIE 7376, Laser Applications in Life Sciences, 73760G (24 November 2010); doi: 10.1117/12.878959; https://doi.org/10.1117/12.878959
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