Microfluidic system for single cell sorting with optical tweezers

Thomas Bruns; Laszlo Becsi; Marc Talkenberg; Michael Wagner; Petra Weber; Ulrich Mescheder; Herbert Schneckenburger

doi:10.1117/12.871450

24 November 2010 Microfluidic system for single cell sorting with optical tweezers

Thomas Bruns, Laszlo Becsi, Marc Talkenberg, Michael Wagner, Petra Weber, Ulrich Mescheder, Herbert Schneckenburger

Proceedings Volume 7376, Laser Applications in Life Sciences; 73760M (2010) https://doi.org/10.1117/12.871450
Event: Laser Applications in Life Sciences 2010, 2010, Oulu, Finland

Abstract

A microfluidic system was developed and combined with optical tweezers for single cell sorting. This system consists of a glass chip of 300 μm thickness with an etched crosswise channel structure, a silicon layer for sealing and a PMMA substrate for tubular coupling. Selected cells are trapped and moved in perpendicular direction to the main flow for recovery in special reservoirs and further evaluation (e.g. by polymerase chain reaction, PCR). In addition, maximum light doses and exposure times for maintaining cell viability were determined.

Citation Download Citation

Thomas Bruns, Laszlo Becsi, Marc Talkenberg, Michael Wagner, Petra Weber, Ulrich Mescheder, and Herbert Schneckenburger "Microfluidic system for single cell sorting with optical tweezers", Proc. SPIE 7376, Laser Applications in Life Sciences, 73760M (24 November 2010); https://doi.org/10.1117/12.871450

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