New staining methods for yeast like fungi under special consideration of human pathogenic fungi

Astrid Paulitsch-Fuchs; Fritz Treiber; Erik Grasser; Walter Buzina; Christian Rosker

doi:10.1117/12.871057

24 November 2010 New staining methods for yeast like fungi under special consideration of human pathogenic fungi

Astrid Paulitsch-Fuchs, Fritz Treiber, Erik Grasser, Walter Buzina, Christian Rosker

Proceedings Volume 7376, Laser Applications in Life Sciences; 73760W (2010) https://doi.org/10.1117/12.871057
Event: Laser Applications in Life Sciences 2010, 2010, Oulu, Finland

Abstract

A new method for in-cellular staining of yeast like fungi with Oregon Green and SYTOX Green is presented enabling their detection as well as the observation of cellular details via confocal laser scanning microscopy. Fluorochromes play an important role in many scientific disciplines including medicine, cell biology and botany. For the visualisation of fungal cell walls Calcofluor White is the flourochrome of choice. The necessity of an UV laser for its excitation makes it unpracticable for daily use. Safranin O, DAPI, 2NBDG, Ethidium Bromide and Acridin-orange are commonly used stains for nuclei in fugal microscopy. The attention was given to the possibility of using the differences in staining patterns to distinguish certain pathogenic yeast species e.g. Candida albicans and Candida krusei. Our results show that high quality microscopy of yeast like organisms can readily be achieved by the use of two suitable fluorochromes.

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Astrid Paulitsch-Fuchs, Fritz Treiber, Erik Grasser, Walter Buzina, and Christian Rosker "New staining methods for yeast like fungi under special consideration of human pathogenic fungi", Proc. SPIE 7376, Laser Applications in Life Sciences, 73760W (24 November 2010); https://doi.org/10.1117/12.871057

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KEYWORDS

Fungi

Yeast

Luminescence

Pathogens

Confocal microscopy

Microscopy

Signal detection

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