Using digital holographic microscopy for 4D tracking of colloid particles

Dmitry Ekimov; Ville Kaikkonen; Anssi Mäkynen

doi:10.1117/12.871449

24 November 2010 Using digital holographic microscopy for 4D tracking of colloid particles

Dmitry Ekimov, Ville Kaikkonen, Anssi Mäkynen

Author Affiliations +

Proceedings Volume 7376, Laser Applications in Life Sciences; 737615 (2010) https://doi.org/10.1117/12.871449
Event: Laser Applications in Life Sciences 2010, 2010, Oulu, Finland

Abstract

A setup and algorithm for 4D tracking of microscopic particles is proposed. The particles in certain volume are detected automatically and their coordinates as well as magnitude and phase distributions saved. The saved data can be used to analyze the number, spatial distribution, size, speed and track of the particles. Calculations show that it is possible to measure the 3D position of a particle having a speed as high as 10 m/s. Experiments from 15 to 2000 fps show that high quality video reconstruction of 1 and 6 μm particle flow is possible at least upto particle density of 200 particles/hologram.

Citation Download Citation

Dmitry Ekimov, Dmitry Ekimov, Ville Kaikkonen, Ville Kaikkonen, Anssi Mäkynen, Anssi Mäkynen, "Using digital holographic microscopy for 4D tracking of colloid particles", Proc. SPIE 7376, Laser Applications in Life Sciences, 737615 (24 November 2010); doi: 10.1117/12.871449; https://doi.org/10.1117/12.871449

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