Paper
22 May 2009 Hybrid FDTD-Fresnel modeling of the scanning confocal microscopy
Bartlomiej Salski, Wojciech Gwarek
Author Affiliations +
Proceedings Volume 7378, Scanning Microscopy 2009; 737826 (2009) https://doi.org/10.1117/12.821824
Event: SPIE Scanning Microscopy, 2009, Monterey, California, United States
Abstract
Scanning confocal microscopy is a widely recognized technique due to its applicability to the imaging of 3D geometries. Image formation in this technique is often analyzed using the Fresnel approximation. However, such an approximation is not sufficient when object dimensions are comparable to the operating wavelength and, most of all, when the target is composed of different semi-transparent materials. Yet, this is very typical for modern integrated circuits where we work with subwavelength features. In such a case target needs to be modeled using full-wave Maxwell theory. However, most of electromagnetic modeling methods (like well established FDTD method) become computationally impractical when the modeled scenario has dimensions measured in hundreds or even thousands of wavelengths like in the far-field microscopy. Therefore, in this paper we propose a hybrid approach that takes advantage of both FDTD and Fresnel approximation methods. The first method will be applied to the modeling of close vicinity of the target. The advantage of that is versatility in definition of arbitrarily shaped geometries as well as wideband approach of the FDTD method. Subsequently, results provided by the FDTD solver will be transferred to the procedure based on the scalar Fresnel approximation used to process the final image pixel by pixel. We will show that the presented method allows imaging of 3D shape of targets proving unique advantage of using FDTD method to the modeling of scanning confocal microscopy.
© (2009) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Bartlomiej Salski and Wojciech Gwarek "Hybrid FDTD-Fresnel modeling of the scanning confocal microscopy", Proc. SPIE 7378, Scanning Microscopy 2009, 737826 (22 May 2009); https://doi.org/10.1117/12.821824
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Cited by 4 scholarly publications.
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KEYWORDS
Finite-difference time-domain method

Confocal microscopy

Image processing

3D modeling

Scattering

3D acquisition

3D image processing

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