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13 July 2009 Photodynamic inactivation of Gram (-) and Gram (+) microorganisms by cationic porphyrins and metalloporphyrins
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Proceedings Volume 7380, Photodynamic Therapy: Back to the Future; 73803I (2009)
Event: 12th World Congress of the International Photodynamic Association, 2009, Seattle, Washington, United States
Photodynamic inactivation (PDI) of microorganisms is successfully applied against Gram (+) microorganisms. However the majority of photosensitizers poorly affect on Gram (-) microorganisms. At present number of works have shown that cationic photosensitizers are able to induce photoinactivation both Gram (+) and Gram (-) microorganisms. The purpose of this work was definition of more effective new cationic pyridylporphyrins with various coordinated metals and functional groups for destruction of Gram (-) microorganisms. The efficiency of new cationic porphyrins and metalloporphyrins (9 compounds) was tested against Gram (-) microorganism E. coli (strain Κ-12). The testing results show high efficiency of metalloporphyrins, especially silver complexes, against E. coli microorganism under dark conditions. 50 % and 100 % cell growth inhibitory concentrations (IC50 and IC100 values, accordingly) of studied metallocomplexes are considerably lower in comparison with metal-free porphyrins. At the same time the Zncomplexes of porphyrins are more phototoxic than their metal-free analogues. Zn-metalloporphyrins with allyl and butyl functional groups were shown the highest efficiency against E. coli. The photodynamic action of cationic Zn -TBut4PyP metalloporphyrin against Gram(+) (St. aureus and St. epidermis) and Gram(-) (E.coli, strain K-12 and Salmonella sp.) microorganisms was investigated. It is revealed, that Gram (+) microorganisms were 3-5 times more susceptible to the compounds' phototoxic influence than Gram (-) microorganisms.
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Grigor V. Gyulkhandanyan, Marina H. Paronyan, Anichka S. Hovsepyan, Robert K. Ghazaryan, Artak G. Tovmasyan, Aram G. Gyulkhandanyan, Anna G. Gyulkhandanyan, and Gayane V. Amelyan "Photodynamic inactivation of Gram (-) and Gram (+) microorganisms by cationic porphyrins and metalloporphyrins", Proc. SPIE 7380, Photodynamic Therapy: Back to the Future, 73803I (13 July 2009);

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