Singlet oxygen kinetics inside living cells: observation of endogenous quencher consumption and consequences for the spatial resolution in time-resolved measurements

Steffen Hackbarth; Jan Schlothauer; Annegret Preuß; Beate Röder

doi:10.1117/12.822830

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13 July 2009 Singlet oxygen kinetics inside living cells: observation of endogenous quencher consumption and consequences for the spatial resolution in time-resolved measurements

Steffen Hackbarth, Jan Schlothauer, Annegret Preuß, Beate Röder

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Proceedings Volume 7380, Photodynamic Therapy: Back to the Future; 738045 (2009) https://doi.org/10.1117/12.822830
Event: 12th World Congress of the International Photodynamic Association, 2009, Seattle, Washington, United States

Abstract

The consumption of singlet oxygen quenchers during illumination of cells incubated with photosensitizers is a serious issue for the determination of singlet oxygen kinetics in cells. Using LNCaP cells incubated with pheophorbide a it will be shown, that already an illumination of 100 nJ/cell dramatically changes the value of the observables. This finding has consequences for the spatial resolution of any time-resolved singlet oxygen measurement, since the radiative rate constant of singlet oxygen in aqueous solutions is very small.

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Steffen Hackbarth, Jan Schlothauer, Annegret Preuß, and Beate Röder "Singlet oxygen kinetics inside living cells: observation of endogenous quencher consumption and consequences for the spatial resolution in time-resolved measurements", Proc. SPIE 7380, Photodynamic Therapy: Back to the Future, 738045 (13 July 2009); https://doi.org/10.1117/12.822830

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