Comparative in vivo study of precursors of PpIX (ALA and MAL) used topically in photodynamic therapy

Raquel Ferreira Rego; Natalia Mayumi Inada; Juliana Ferreira; Fernando Manuel Araújo-Moreira; Vanderlei Salvador Bagnato

doi:10.1117/12.823012

13 July 2009 Comparative in vivo study of precursors of PpIX (ALA and MAL) used topically in photodynamic therapy

Raquel Ferreira Rego, Natalia Mayumi Inada, Juliana Ferreira, Fernando Manuel Araújo-Moreira, Vanderlei Salvador Bagnato

Author Affiliations +

Proceedings Volume 7380, Photodynamic Therapy: Back to the Future; 73805I (2009) https://doi.org/10.1117/12.823012
Event: 12th World Congress of the International Photodynamic Association, 2009, Seattle, Washington, United States

Abstract

The efficacy of Photodynamic Therapy (PDT) combined with aminolevulinic acid (ALA) or methyl aminolevulinate (MAL) in treatment of cancer has been studied for over ten years. However, there is no established dose for the topical use of these drugs in PDT. The purpose of this study was the comparison of induced PDT response of ALAsense (5-aminolevulinic acid - ALA) and Metvix (methyl aminolevulinate - MAL). Depth of necrosis induced by PDT was analyzed in normal liver of male Wistar rats, using different light doses and topical application of both PpIX precursors - ALA and MAL. PDT was performed with a diode laser at 630 nm with different doses of light (20, 50, 100 and 200 J/cm²), and intensity of 250 mW/cm². Depth of necrosis analysis was used to calculate the threshold dose for each drug. The results showed that MAL-PDT presented a better response than ALA-PDT, mainly due to formulation differences. Moreover, the ability of the ALA PpIX production was more efficient.

Citation Download Citation

Raquel Ferreira Rego, Natalia Mayumi Inada, Juliana Ferreira, Fernando Manuel Araújo-Moreira, and Vanderlei Salvador Bagnato "Comparative in vivo study of precursors of PpIX (ALA and MAL) used topically in photodynamic therapy", Proc. SPIE 7380, Photodynamic Therapy: Back to the Future, 73805I (13 July 2009); https://doi.org/10.1117/12.823012

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available