14 July 2009 In vivo quantification of mTHPC fluorescence in skinfold observation chamber using excitation and detection towards the near infrared region
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Proceedings Volume 7380, Photodynamic Therapy: Back to the Future; 738063 (2009) https://doi.org/10.1117/12.822947
Event: 12th World Congress of the International Photodynamic Association, 2009, Seattle, Washington, United States
Abstract
In this study, a ratiometric quantification method is developed and applied to monitor mesotetra(hydroxyphenyl) chlorin (mTHPC) pharmacokinetics in the rat skin-fold observation chamber. The method employs a combination of dual-wavelength excitation and dual-wavelength detection. The excitation and detection wavelengths were selected in close to NIR. The first excitation wavelength was used to excite the mTHPC and autofluorescence and the second to excite only autofluorescence, so that this could be substracted. Subsequently the difference was divided by the autofluorescence. Since the method applies division of signal with no mTHPC fluorescence, theory suggests on linear dependency of the method on photosensitizer concentration.
© (2009) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Slávka Kascáková, Slávka Kascáková, Sebastiaan de Visscher, Sebastiaan de Visscher, Bastiaan Kruijt, Bastiaan Kruijt, Henriëtte S. de Bruijn, Henriëtte S. de Bruijn, Angélique van der Ploeg-van den Heuvel, Angélique van der Ploeg-van den Heuvel, Henricus J. C. M. Sterenborg, Henricus J. C. M. Sterenborg, Max J. H. Witjes, Max J. H. Witjes, Arjen Amelink, Arjen Amelink, Dominic J. Robinson, Dominic J. Robinson, } "In vivo quantification of mTHPC fluorescence in skinfold observation chamber using excitation and detection towards the near infrared region", Proc. SPIE 7380, Photodynamic Therapy: Back to the Future, 738063 (14 July 2009); doi: 10.1117/12.822947; https://doi.org/10.1117/12.822947
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