5 August 2009 Surface-enhanced Raman scattering detection of amino acids and peptides in a microfluidic device
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Proceedings Volume 7386, Photonics North 2009; 73860A (2009); doi: 10.1117/12.839790
Event: Photonics North 2009, 2009, Quebec, Canada
Abstract
Here we report the results of investigations of Surface Enhanced Raman Scattering (SERS) from amino acids and peptides. In order to obtain optimum signals a standard microfluidic chip has been modified with the help of laser micromachining technique to increase scattering light collection efficiency. We have studied the SERS signals from the following amino acids: tryptophan (Trp), phenylalanine (Phe) and glycine (Gly) and peptides Trp-Trp and Gly-Gly-Gly. The optimum conditions for observing the spectrum from these amino acids and peptides have determined. In our studies the highest enhancement observed is from the amino acid Trp. Large signal enhancements were observed and the lowest detectable concentration of Trp was estimated to 4·10 -9 M.
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A. Kandakkathara, I. Utkin, R. Fedosejevs, "Surface-enhanced Raman scattering detection of amino acids and peptides in a microfluidic device", Proc. SPIE 7386, Photonics North 2009, 73860A (5 August 2009); doi: 10.1117/12.839790; http://dx.doi.org/10.1117/12.839790
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KEYWORDS
Microfluidics

Raman spectroscopy

Surface enhanced Raman spectroscopy

Raman scattering

Silver

Scattering

Laser scattering

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