Three-dimensional image contrast using biospeckle

Robson Pierangeli Godinho; Roberto A. Braga Jr.

doi:10.1117/12.870719

13 September 2010 Three-dimensional image contrast using biospeckle

Robson Pierangeli Godinho, Roberto A. Braga Jr.

Proceedings Volume 7387, Speckle 2010: Optical Metrology; 738718 (2010) https://doi.org/10.1117/12.870719
Event: Speckle 2010, 2010, Florianapolis, Brazil

Abstract

The biospeckle laser (BSL) has been applied in many areas of knowledge and a variety of approaches has been presented to address the best results in biological and non-biological samples, in fast or slow activities, or else in defined flow of materials or in random activities. The methodologies accounted in the literature consider the apparatus used in the image assembling and the way the collected data is processed. The image processing steps presents in turn a variety of procedures with first or second order statistics analysis, and as well with different sizes of data collected. One way to access the biospeckle in defined flow, such as in capillary blood flow in alive animals, was the adoption of the image contrast technique which uses only one image from the illuminated sample. That approach presents some problems related to the resolution of the image, which is reduced during the image contrast processing. In order to help the visualization of the low resolution image formed by the contrast technique, this work presents the three-dimensional procedure as a reliable alternative to enhance the final image. The work based on a parallel processing, with the generation of a virtual map of amplitudes, and maintaining the quasi-online characteristic of the contrast technique. Therefore, it was possible to generate in the same display the observed material, the image contrast result and in addiction the three-dimensional image with adjustable options of rotation. The platform also offers to the user the possibility to access the 3D image offline.

Citation Download Citation

Robson Pierangeli Godinho and Roberto A. Braga Jr. "Three-dimensional image contrast using biospeckle", Proc. SPIE 7387, Speckle 2010: Optical Metrology, 738718 (13 September 2010); https://doi.org/10.1117/12.870719

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