20 August 2009 The interaction of Escherichia coli with its surrounding three dimensional substrate measured by oscillatory optical tweezers
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Abstract
It has been known that the shape, the locomotion, and the growth of cells and bacteria are often affected by their interactions with extra cellular matrix (ECM). However, it is difficult to quantify such interactions with conventional biochemical methods. In this paper we report the application of oscillatory optical tweezers to trap and oscillate three types of E. coli, in 0.2% LB agar substrate to quantify the E. coli - substrate interactions in terms of the elasticity modulus G'. The three types of E. coli are BW25113 (wild-type, normal with flagellum), BW25113 (normal with flagellum, but subjected to UV light exposure for 1 hr to deactivate the flagellum), and JW1923 (a null-flagellum mutant of BW25113). Our results indicate that the value of G' for the later two is significantly higher than that for the normal wild-type (WT). We speculated that the interaction with the surrounding is perturbed, and hence reduced, mainly by the motion of the flagellum in the case of the WT.
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Pei-Wen Yen, Pei-Wen Yen, Pin-Tzu Su, Pin-Tzu Su, Li-Jen Hung, Li-Jen Hung, Ming-Tzo Wei, Ming-Tzo Wei, Wan-Jr Syu, Wan-Jr Syu, Arthur Chiou, Arthur Chiou, } "The interaction of Escherichia coli with its surrounding three dimensional substrate measured by oscillatory optical tweezers", Proc. SPIE 7400, Optical Trapping and Optical Micromanipulation VI, 74000C (20 August 2009); doi: 10.1117/12.829880; https://doi.org/10.1117/12.829880
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