20 August 2009 Measuring droplet properties through passive microrheology in optical tweezers
Author Affiliations +
We demonstrate the use of holographic optical tweezers to form arrangements of silica beads for trapping and measuring the mechanical properties of micron sized objects, such as oil droplets and yeast cells. This allows us to investigate the mechanical properties of the constrained object, which need not be optically trapped itself (thus preventing radiation damage and allowing objects with a low refractive index to be constrained). By compressing the object with the beads we are able to determine the size of the trapped object and show that there is an elastic coupling between the beads due to the presence of a trapped object. We expect more detailed analysis of the system will allow mechanical and frequency-dependent viscoelastic properties of objects to be investigated.
© (2009) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
R. W. Bowman, R. W. Bowman, G. M. Gibson, G. M. Gibson, A. M. Yao, A. M. Yao, M. Tassieri, M. Tassieri, J. M. Cooper, J. M. Cooper, M. J. Padgett, M. J. Padgett, } "Measuring droplet properties through passive microrheology in optical tweezers", Proc. SPIE 7400, Optical Trapping and Optical Micromanipulation VI, 740015 (20 August 2009); doi: 10.1117/12.828139; https://doi.org/10.1117/12.828139

Back to Top