Hyperbranched polysiloxysilane nanoparticles for nonviral gene delivery vectors and nanoprobes

Won Jin Kim; Adela C. Bonoiu; Kwang-Sup Lee; Teruaki Hayakawa; Cheng Xia; Masa-aki Kakimoto; Haridas E. Pudavar; Paras N. Prasad

doi:10.1117/12.824518

20 August 2009 Hyperbranched polysiloxysilane nanoparticles for nonviral gene delivery vectors and nanoprobes

Won Jin Kim, Adela C. Bonoiu, Kwang-Sup Lee, Teruaki Hayakawa, Cheng Xia, Masa-aki Kakimoto, Haridas E. Pudavar, Paras N. Prasad

Author Affiliations +

Proceedings Volume 7403, Nanobiosystems: Processing, Characterization, and Applications II; 74030H (2009) https://doi.org/10.1117/12.824518
Event: SPIE NanoScience + Engineering, 2009, San Diego, California, United States

Abstract

We report an approach to produce predefined surface charge tunable gene delivery vectors using siloxysilsane-based polymer for gene delivery studies. To obtain nonviral vectors, new series of hyperbranched polysiloxysilane (HBPS) were synthesized, and the end groups in polymer structures have modified with hydrophilic molecules; in other words, carboxylic acid and quaternary ammonium groups were employed into terminal structures to give the amphiphilicity. The novelty of these amphiphilic HBPS polymers lies in the fact that nanoparticles with different zeta potential (surface charge density) can be easily tailored and functionalized. These polymeric nanoparticles which containing various chemical groups on the surface indicated altered surface charge distributions (from -40 to +64mV). Finally, the use of these nanoparticles as efficient gene delivery vectors was demonstrated by means of in vitro transfection study using β- galactosidase plasmid and pEGFP-N1 plasmid, and the most efficient combination was obtained using HBPS-CN30:70.

Citation Download Citation

Won Jin Kim, Adela C. Bonoiu, Kwang-Sup Lee, Teruaki Hayakawa, Cheng Xia, Masa-aki Kakimoto, Haridas E. Pudavar, and Paras N. Prasad "Hyperbranched polysiloxysilane nanoparticles for nonviral gene delivery vectors and nanoprobes", Proc. SPIE 7403, Nanobiosystems: Processing, Characterization, and Applications II, 74030H (20 August 2009); https://doi.org/10.1117/12.824518

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