20 August 2009 Nano-engineering of materials for nonlinear optical imaging
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Abstract
We discuss the use of genetically modified fluorescent proteins (FPs) and a specially engineered chromophore for nonlinear optical imaging. While it is clear that FPs can be used for two-photon fluorescence (TPF) microscopy, we show that they also exhibit a large second-order nonlinear optical response, useful for second-harmonic generation (SHG) imaging. The relation between the linear and nonlinear optical properties in a small series of FPs will be discussed. We also present a new optimized chromophore for combined TPF and SHG microscopy and we show imaging results obtained on this chromophore on a water droplet model system.
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Koen Clays, Jos Vanderleyden, Harry L Anderson, "Nano-engineering of materials for nonlinear optical imaging", Proc. SPIE 7413, Linear and Nonlinear Optics of Organic Materials IX, 74130J (20 August 2009); doi: 10.1117/12.824517; https://doi.org/10.1117/12.824517
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