20 August 2009 Nano-engineering of materials for nonlinear optical imaging
Author Affiliations +
We discuss the use of genetically modified fluorescent proteins (FPs) and a specially engineered chromophore for nonlinear optical imaging. While it is clear that FPs can be used for two-photon fluorescence (TPF) microscopy, we show that they also exhibit a large second-order nonlinear optical response, useful for second-harmonic generation (SHG) imaging. The relation between the linear and nonlinear optical properties in a small series of FPs will be discussed. We also present a new optimized chromophore for combined TPF and SHG microscopy and we show imaging results obtained on this chromophore on a water droplet model system.
© (2009) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Koen Clays, Koen Clays, Jos Vanderleyden, Jos Vanderleyden, Harry L Anderson, Harry L Anderson, } "Nano-engineering of materials for nonlinear optical imaging", Proc. SPIE 7413, Linear and Nonlinear Optics of Organic Materials IX, 74130J (20 August 2009); doi: 10.1117/12.824517; https://doi.org/10.1117/12.824517

Back to Top