Fast Analog Technique For Determining Fluorescence Lifetimes Of Multicomponent Materials By Pulsed Laser

W. L. Borst; S. Gangopadhyay; M. W. Pleil

doi:10.1117/12.966920

1 January 1987 Fast Analog Technique For Determining Fluorescence Lifetimes Of Multicomponent Materials By Pulsed Laser

W. L. Borst, S. Gangopadhyay, M. W. Pleil

Proceedings Volume 0743, Fluorescence Detection; (1987) https://doi.org/10.1117/12.966920
Event: OE LASE'87 and EO Imaging Symposium, 1987, Los Angeles, CA, United States

Abstract

This paper describes a system capable of measuring fluorescent lifetimes and time-resolved spectra of microscopic particles, thus substantially enhancing the usefulness of fluorescence microscopy as a characterizing tool. Tests and applications of this technique for identifying component lifetimes (in the nanosecond and subnanosecond range) and spectra in organic model compounds also are described. A multi-exponential decay fit to the data yields fluorescence decay times, percentage contributions (fractional intensities) and time-resolved spectra for each decay. Two-component non-interacting mixtures are resolved if the decay times differ by a factor of two and the percentage contribution from each fluorophore is more than 10%. For three-component mixtures, it is necessary for the resolution of the lifetimes that the decay times differ by at least a factor of three and the percentage contribution from each component be more than 15%.

Citation Download Citation

W. L. Borst, S. Gangopadhyay, and M. W. Pleil "Fast Analog Technique For Determining Fluorescence Lifetimes Of Multicomponent Materials By Pulsed Laser", Proc. SPIE 0743, Fluorescence Detection, (1 January 1987); https://doi.org/10.1117/12.966920

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