Molecular Dynamics Studies Using Frequency-Domain Fluorometry

Enrico Gratton; Beniamino Barbieri

doi:10.1117/12.966933

1 January 1987 Molecular Dynamics Studies Using Frequency-Domain Fluorometry

Enrico Gratton, Beniamino Barbieri

Proceedings Volume 0743, Fluorescence Detection; (1987) https://doi.org/10.1117/12.966933
Event: OE LASE'87 and EO Imaging Symposium, 1987, Los Angeles, CA, United States

Abstract

The dynamical behavior of large molecules can be studied by measuring the fluorescence of fluorophores bound to the molecule. Time-resolved fluorescence, using frequency-domain technique, provides a unique tool for the analysis of the emission from complex systems. Time resolution of few picoseconds is attainable using state-of-the-art commercial phase and modulation fluorometers. The development of these high-resolution instruments allowed for a new understanding of the internal motions of proteins and of molecules embedded in natural and synthetic membranes.

Citation Download Citation

Enrico Gratton and Beniamino Barbieri "Molecular Dynamics Studies Using Frequency-Domain Fluorometry", Proc. SPIE 0743, Fluorescence Detection, (1 January 1987); https://doi.org/10.1117/12.966933

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