A micro fluorescent activated cell sorter for astrobiology applications

Donald W. Platt; Richard B. Hoover

doi:10.1117/12.831336

3 September 2009 A micro fluorescent activated cell sorter for astrobiology applications

Donald W. Platt, Richard B. Hoover

Proceedings Volume 7441, Instruments and Methods for Astrobiology and Planetary Missions XII; 74410B (2009) https://doi.org/10.1117/12.831336
Event: SPIE Optical Engineering + Applications, 2009, San Diego, California, United States

Abstract

A micro-scale Fluorescent Activated Cell Sorter (μFACS) for astrobiology applications is under development. This device is designed to have a footprint of 7 cm x 7 cm x 4 cm and allow live-dead counts and sorting of cells that have fluorescent characteristics from staining. The μFACS system takes advantage of microfludics to create a cell sorter that can fit in the palm of the hand. A micron-scale channel allows cells to pass by a blue diode which causes emission of marker-expressed cells which are detected by a filtered photodetector. A small microcontroller then counts cells and operates high speed valves to select which chamber the cell is collected in (a collection chamber or a waste chamber). Cells with the expressed characteristic will be collected in the collection chamber. This system has been built and is currently being tested. We are also designing a system with integrated MEMS-based pumps and valves for a small and compact unit to fly on small satellite-based biology experiments.

Citation Download Citation

Donald W. Platt and Richard B. Hoover "A micro fluorescent activated cell sorter for astrobiology applications", Proc. SPIE 7441, Instruments and Methods for Astrobiology and Planetary Missions XII, 74410B (3 September 2009); https://doi.org/10.1117/12.831336

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