3 September 2009 A micro fluorescent activated cell sorter for astrobiology applications
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Abstract
A micro-scale Fluorescent Activated Cell Sorter (μFACS) for astrobiology applications is under development. This device is designed to have a footprint of 7 cm x 7 cm x 4 cm and allow live-dead counts and sorting of cells that have fluorescent characteristics from staining. The μFACS system takes advantage of microfludics to create a cell sorter that can fit in the palm of the hand. A micron-scale channel allows cells to pass by a blue diode which causes emission of marker-expressed cells which are detected by a filtered photodetector. A small microcontroller then counts cells and operates high speed valves to select which chamber the cell is collected in (a collection chamber or a waste chamber). Cells with the expressed characteristic will be collected in the collection chamber. This system has been built and is currently being tested. We are also designing a system with integrated MEMS-based pumps and valves for a small and compact unit to fly on small satellite-based biology experiments.
© (2009) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Donald W. Platt, Donald W. Platt, Richard B. Hoover, Richard B. Hoover, } "A micro fluorescent activated cell sorter for astrobiology applications", Proc. SPIE 7441, Instruments and Methods for Astrobiology and Planetary Missions XII, 74410B (3 September 2009); doi: 10.1117/12.831336; https://doi.org/10.1117/12.831336
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