SLM-based phase-contrast filtering for single and multiple image acquisition

Michael Warber; Susanne Zwick; Malte Hasler; Tobias Haist; Wolfgang Osten

doi:10.1117/12.825945

24 August 2009 SLM-based phase-contrast filtering for single and multiple image acquisition

Michael Warber, Susanne Zwick, Malte Hasler, Tobias Haist, Wolfgang Osten

Proceedings Volume 7442, Optics and Photonics for Information Processing III; 74420E (2009) https://doi.org/10.1117/12.825945
Event: SPIE Optical Engineering + Applications, 2009, San Diego, California, United States

Abstract

We report on the implementation of different phase contrast methods using an SLM in a microscope. The ease of generating complex phase filters with the SLM opens the possibility to realize standard filters adapted to the specimen and the possibility to develop new phase contrasting methods. Due to the real-time addressing we can obtain a number of different images from each specimen recorded nearly simultaneously with the same microscope objective. We demonstrate how to realize different versions of differential interference contrast imaging, Zernike-type imaging and dark-field imaging and the combination of the different images by simple post processing. Experimental results for biological as well as technical specimens are presented.

Citation Download Citation

Michael Warber, Susanne Zwick, Malte Hasler, Tobias Haist, and Wolfgang Osten "SLM-based phase-contrast filtering for single and multiple image acquisition", Proc. SPIE 7442, Optics and Photonics for Information Processing III, 74420E (24 August 2009); https://doi.org/10.1117/12.825945

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