Multifocus CARS microscopy for realtime vibrational imaging

Mamoru Hashimoto; Takeo Minamikawa; Hirohiko Niioka; Tsutomu Araki

doi:10.1117/12.838084

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2 December 2009 Multifocus CARS microscopy for realtime vibrational imaging

Mamoru Hashimoto, Takeo Minamikawa, Hirohiko Niioka, Tsutomu Araki

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Proceedings Volume 7507, 2009 International Conference on Optical Instruments and Technology: Optical Trapping and Microscopic Imaging; 75070H (2009) https://doi.org/10.1117/12.838084
Event: International Conference on Optical Instrumentation and Technology, 2009, Shanghai, China

Abstract

We developed a multifocus excitation coherent anti-Stokes Raman scattering microscope using a microlens array scanner for realtime molecular imaging. Two picoseond mode-locked lasers tightly synchronized were splited to a few tens of foci with the microlens array, the foci excited the sample parallely and the generated CARS from each spot was detected with an image sensor at once. By the multifocus excitation, exposure time was prolonged proportionally to the number of the foci because of parallel excitation and detection. The video-rate (frame rate of 30 fps) imaging of polystyrene beads in water was demonstrated, and the Brownian motion of beads were clearly obtained. The three-dimensional reconstructed imaging of living HeLa cells (frame rate of 5 fps, 85 images) was also demonstrated.

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Mamoru Hashimoto, Takeo Minamikawa, Hirohiko Niioka, and Tsutomu Araki "Multifocus CARS microscopy for realtime vibrational imaging", Proc. SPIE 7507, 2009 International Conference on Optical Instruments and Technology: Optical Trapping and Microscopic Imaging, 75070H (2 December 2009); https://doi.org/10.1117/12.838084

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