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2 December 2009Lysosomal photodamage induces cell death via mitochondrial apoptotic pathway
Lysosomal photosensitizers have been used in photodynamic therapy (PDT). Combination of such photosensitizers and
light causes lysosomal photodamage, inducing cell death. The lysosomal disruption can lead to apoptosis but its
signaling pathways remain to be elucidated. In this study, we selected N-aspartyl chlorin e6 (NPe6), an effective
photosensitizer which preferentially accumulates in lysosomes, to study the mechanism of apoptosis caused by
lysosomal photodamage. Apoptosis in living human lung adenocarcinoma cells treated by NPe6-PDT was studied using
real-time single-cell analysis. In this study, the fluorescence probes Cyto c-GFP and DsRed-Mit were used to detect the
spatial and temporal changes of cytochrome c in real-time in sub-cell level; the Rhodamine 123 dyes were used to
monitor the changes of mitochondrial membrane potential. The results showed that, after PDT treatment,the
mitochondrial membrane potential decreased, and cytochrome c released from mitochondria; The caspase-3 was
activated obviously. These results suggested that lysosomal photodamage activates mitochondrial apoptotic pathway to
induce cell death.
Lei Liu,Xian-wang Wang, andHui Li
"Lysosomal photodamage induces cell death via mitochondrial apoptotic pathway", Proc. SPIE 7507, 2009 International Conference on Optical Instruments and Technology: Optical Trapping and Microscopic Imaging, 75070I (2 December 2009); https://doi.org/10.1117/12.838110
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Lei Liu, Xian-wang Wang, Hui Li, "Lysosomal photodamage induces cell death via mitochondrial apoptotic pathway," Proc. SPIE 7507, 2009 International Conference on Optical Instruments and Technology: Optical Trapping and Microscopic Imaging, 75070I (2 December 2009); https://doi.org/10.1117/12.838110