20 November 2009 Light scattering modeling of bacteria using spheroids and cylinders
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Numerical simulations of light scattering by irregularly shaped bacteria are carried out using the T-matrix method. A previously developed T-matrix code for the study of light scattering by randomly oriented non-spherical particles is used for the current purpose and it is validated against Mie-theory using coccus. Simplified particle shapes of spheroids and cylinders for simulating scattering by irregularly shaped bacteria are studied. The results for the angular distributions of the scattering matrix elements of B.Subtilis at wavelength 0.6328μm are presented. Their dependence on shape and model are discussed. Analysis suggests that spheroids perform better than cylinders for B.Subtilis. Calculations of the scatter matrix elements to determine bacteria sizes as well as shapes may be an accurate method and may be used to determine what the bacteria are.
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Chunxia Feng, Chunxia Feng, Lihua Huang, Lihua Huang, Jie Han, Jie Han, Guangchao Zhou, Guangchao Zhou, Aijun Zeng, Aijun Zeng, Yongkai Zhao, Yongkai Zhao, Huijie Huang, Huijie Huang, } "Light scattering modeling of bacteria using spheroids and cylinders", Proc. SPIE 7511, 2009 International Conference on Optical Instruments and Technology: Optoelectronic Measurement Technology and Systems, 75110P (20 November 2009); doi: 10.1117/12.837919; https://doi.org/10.1117/12.837919

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