A new method of non-destructive quality inspection of fruits was investigated based on multi-spectral imaging technology, and the system was also developed, which consists of lighting chamber, multi-spectral light source, CCD camera, frame grabber and a computer. According to the basic color theory; multi-spectral images were abstained by this system, and analyzed using multi-spectral images fusion methods. It was found that the multi-spectral images had the highest recognition rate when the spectral bands of multi-spectral fusion images are red spectral band, blue spectral
band, near infrared spectral band. After the feature extraction of the multi-spectral images, the fruits were classified according to size, shape, defects and other parameters. It was shown by the experiment that the accuracy of the fruits quality inspection can be achieved 90.25% using multi-spectral detection technology on non-destructive.