Probing of marker proteins in cancer tissue using quantum dots with Hadamard transform fluorescence imaging microscopy

Hao Xu; Chuang Chen; Yan Li; Hong-Wu Tang

doi:10.1117/12.841819

28 October 2009 Probing of marker proteins in cancer tissue using quantum dots with Hadamard transform fluorescence imaging microscopy

Hao Xu, Chuang Chen, Yan Li, Hong-Wu Tang

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Proceedings Volume 7519, Eighth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2009); 751917 (2009) https://doi.org/10.1117/12.841819
Event: Photonics and Optoelectronics Meetings 2009, 2009, Wuhan, China

Abstract

A domestic-made Hadamard transform spectral imaging microscope was employed to provide high-resolutional fluorescence spectrum and image of tiny samples such as single cells and tissues. By using agron laser line at 454 nm to excite fluorescence and based on immunostaining with quantum dots (QDs) at different wavelengths to tag and trace breast cancer biomarkers in human breast cancer tissues, in situ single-color and dual-color fluorescence imaging for human epidermal growth factor receptor 2 (HER2), estrogen receptor (ER) and proliferating cell nuclear antigen (PCNA) in tissues were realized, by using the Hadamard imaging microscope to capture the high S/N ratio fluorescence images. Moreover, through the comparative study of the differences between fluorescence spectra and images of positive samples and negative control, a method was proposed to evaluate tumor malignancy of the specimens based on the analysis of distribution of HER2, ER and PCNA in the tissues. The results show that the Hadamard transform spectral imaging technique can be applied to visualize and quantitatively measure the subcellular molecules inside the tumor tissues and has great potential in biology and medical diagnosis.

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Hao Xu, Chuang Chen, Yan Li, and Hong-Wu Tang "Probing of marker proteins in cancer tissue using quantum dots with Hadamard transform fluorescence imaging microscopy", Proc. SPIE 7519, Eighth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2009), 751917 (28 October 2009); https://doi.org/10.1117/12.841819

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KEYWORDS

Luminescence

Tissues

Proteins

Breast cancer

Tumors

Imaging spectroscopy

Microscopes

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