28 October 2009 Probing of marker proteins in cancer tissue using quantum dots with Hadamard transform fluorescence imaging microscopy
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Abstract
A domestic-made Hadamard transform spectral imaging microscope was employed to provide high-resolutional fluorescence spectrum and image of tiny samples such as single cells and tissues. By using agron laser line at 454 nm to excite fluorescence and based on immunostaining with quantum dots (QDs) at different wavelengths to tag and trace breast cancer biomarkers in human breast cancer tissues, in situ single-color and dual-color fluorescence imaging for human epidermal growth factor receptor 2 (HER2), estrogen receptor (ER) and proliferating cell nuclear antigen (PCNA) in tissues were realized, by using the Hadamard imaging microscope to capture the high S/N ratio fluorescence images. Moreover, through the comparative study of the differences between fluorescence spectra and images of positive samples and negative control, a method was proposed to evaluate tumor malignancy of the specimens based on the analysis of distribution of HER2, ER and PCNA in the tissues. The results show that the Hadamard transform spectral imaging technique can be applied to visualize and quantitatively measure the subcellular molecules inside the tumor tissues and has great potential in biology and medical diagnosis.
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Hao Xu, Hao Xu, Chuang Chen, Chuang Chen, Yan Li, Yan Li, Hong-Wu Tang, Hong-Wu Tang, } "Probing of marker proteins in cancer tissue using quantum dots with Hadamard transform fluorescence imaging microscopy", Proc. SPIE 7519, Eighth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2009), 751917 (28 October 2009); doi: 10.1117/12.841819; https://doi.org/10.1117/12.841819
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