28 October 2009 Protein kinase C δ promotes cell apoptosis induced by high fluence low-power laser irradiation
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Abstract
Protein kinase C delta (PKCδ) mediates apoptosis downstream of many apoptotic stimuli. However, whether PKCδ is involved in high fluence low-power laser irradiation (HF-LPLI)-induced cell apoptosis is still not understood. In this study, we investigated the activity of PKCδ in response to HF-LPLI treatment in human breast adenocarcinoma cells (MCF-7). Cells were transiently transfected pGFP-PKCδ and irradiated with a He-Ne laser for 10 minutes under a fluence of 120 J/cm2 after 36 hours expression. Nuclear staining with Hoechst 33258 and analysis of flow cytometry based on Annexin V-FITC/PI revealed that overexpression of PKCδ promoted the apoptotic effect of HF-LPLI. Taken together, we demonstrated that PKCδ promoted cell apoptosis caused by HF-LPLI, providing a new insight of mechanisms of HF-LPLI.
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Heng Zhang, Heng Zhang, Da Xing, Da Xing, Shengnan Wu, Shengnan Wu, Xuegang Sun, Xuegang Sun, } "Protein kinase C δ promotes cell apoptosis induced by high fluence low-power laser irradiation", Proc. SPIE 7519, Eighth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2009), 751919 (28 October 2009); doi: 10.1117/12.841566; https://doi.org/10.1117/12.841566
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