28 October 2009 Raman spectral study of anti-angiogenic drugs on the role of chick vascular
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Abstract
Inhibit angiogenesis is one of the important tumor therapy. If the mechanism of vascular changes can be detected at molecular level, it will have therapeutic significance. Raman spectroscopy, which can be applied to the structural analysis of solid, liquid or solution of biological molecules, is a non-destructive spectral technology holding very rich information. Basing on Confocal Raman Microscope, a unique system is developed for obtaining the different Raman spectra of the chick embryo vascular with the anti-angiogenic drugs - thalidomide and without. In the study, the location and shape of the average Raman spectra of vessels in drug 5h were very similar to the ones without medicine, and the intensity of some characteristic peaks changed, such as 1441cm-1,1527cm-1 and 1657cm-1 showing markedly increasing, while the 971cm-1 and 1081cm-1 decreasing. This change was due to anti- angiogenic drugs that caused the nucleic acid, protein, phospholipids, and other important biological molecules of the vessels on the structure or content tovary. PCA was used to distinguish between the two kinds of vascular with the result that they were accurately partitioned.The study indicated that Raman spectroscopy could be an effective tool for detection of the mechanism of vascular changes.
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Ruixiang Huang, Ruixiang Huang, Qisong Chen, Qisong Chen, Shaojun Lin, Shaojun Lin, Chao Li, Chao Li, } "Raman spectral study of anti-angiogenic drugs on the role of chick vascular", Proc. SPIE 7519, Eighth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2009), 75191F (28 October 2009); doi: 10.1117/12.843938; https://doi.org/10.1117/12.843938
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