A growing body of literature indicated the cytosolic free Ca2+ concentration of skeletal muscle cells changes significantly
during exercise-induced fatigue. But it is confusing whether cytosolic free Ca2+ concentration increase or decrease.
Furthermore, current researches mainly adopt muscle tissue homogenate as experiment material, but the studies based on
cellular and subcellular level is seldom. This study is aimed to establish rat skeletal muscle cell model of
exercise-induced fatigue, and confirm the change of cytosolic free Ca2+ concentration of skeletal muscle cells in rats preand
post- exercise-induced fatigue. In this research, six male Wistar rats were randomly divided into two groups: control
group (n=3) and exercise-induced fatigue group (n=3). The former group were allowed to freely move and the latter were
forced to loaded swimming to exhaustive. Three days later, all the rats were sacrificed, the muscle tissue from the same
site of skeletal muscle were taken out and digested to cells. After primary culture of the two kinds of skeletal muscle
cells from tissue, a fluorescent dye-Fluo-3 AM was used to label the cytosolic free Ca2+. The fluorescent of Ca2+ was
recorded by confocal laser scanning microscopy. The results indicated that, the Ca2+ fluorescence intensity of cells from
the rat of exercise-induced fatigue group was significantly higher than those in control group. In conclusion, cytosolic
free Ca2+ concentration of skeletal muscle cells has a close relation with exercise-induced fatigue, and the increase of
cytosolic free Ca2+ concentration may be one of the important factors of exercise-induced fatigue.