The biocompatibility of the dermal injection of glycerol in vivo to achieve optical clearing

Zongzhen Mao; Xiang Wen; Jing Wang; Dan Zhu

doi:10.1117/12.843506

28 October 2009 The biocompatibility of the dermal injection of glycerol in vivo to achieve optical clearing

Zongzhen Mao, Xiang Wen, Jing Wang, Dan Zhu

Proceedings Volume 7519, Eighth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2009); 75191N (2009) https://doi.org/10.1117/12.843506
Event: Photonics and Optoelectronics Meetings 2009, 2009, Wuhan, China

Abstract

Glycerol has been proved to be the best optical clearing agent on skin, but the ideal optical clearing effect can only be achieved by injecting glycerol underneath the skin. As a result of our former research, high concentrations of glycerol injection may lead unrecoverable injury on skin, while the injecting of 30% glycerol solution can achieve good clearing effect and be recovered for macro observation. In order to get further investigation of the biocompatibility on skin for 30% glycerol solution, skin samples were achieved 10 min, 30 min, 24 hour and 10 day after the injection of 30% glycerol solution. The samples were observed by optical microscopy with HE staining and electron microscopy separately. Also, the Laser Speckle Contrast Imaging (LSCI) technique was used to monitor the influence of glycerol on rat dermal vessels. The result shows the thickness of the dermal layer or the diameters of collagen fibers have no significant changes, and obvious injury like swell or fracture haven't been observed on fibers. From the dermal side, it can be clearly seen that arteriole and venule turned clearer. The blood flow in vessels appears to be slowing down or stopped in a short time, and recovered in 16 min. The results represent for dermal injection of optical clearing agents, 30% glycerol solution is an ideal agent.

Citation Download Citation

Zongzhen Mao, Xiang Wen, Jing Wang, and Dan Zhu "The biocompatibility of the dermal injection of glycerol in vivo to achieve optical clearing", Proc. SPIE 7519, Eighth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2009), 75191N (28 October 2009); https://doi.org/10.1117/12.843506

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available