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14 April 2010 Measurement and control of motion of nanoparticles in microchannel
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Proceedings Volume 7522, Fourth International Conference on Experimental Mechanics; 75221N (2010) https://doi.org/10.1117/12.851444
Event: Fourth International Conference on Experimental Mechanics, 2009, Singapore, Singapore
Abstract
In microbiochemical analysis, the concentration of biomaterials near a wall greatly affects the detection and reaction efficiency. In this paper, we evaluate the interaction between nanoparticles and a microchannel wall. We measured the concentration distribution of 100-nm-diameter particles, which are equivalent in size to viruses, and attempted to cause nanoparticles to accumulate near the wall by applying an electric field with the aim of improving the reaction efficiency. We used total internal reflection fluorescence microscopy to measure the 3D particle distribution. This technique employs evanescent light (the intensity of which decays exponentially with distance) as illumination. By exploiting the characteristics of evanescent light, we were able to determine the 3D-position of a particle from its luminescence. The results reveal that there are very low concentrations of particles near a channel wall due to the potential barrier between the particles and the wall. This implies that immune reactions will not occur effectively near walls. Subsequently, we tried to concentrate nanoparticles near the wall by applying an electric field to the suspension. The particles moved toward the wall and became attached to it, overcoming the potential barrier. We anticipate that applying this method to biochemical analysis will greatly increase reaction efficiencies.
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Takuma Mizukoshi, Kensuke Kanda, Satoshi Ogata, and Ming Yang "Measurement and control of motion of nanoparticles in microchannel", Proc. SPIE 7522, Fourth International Conference on Experimental Mechanics, 75221N (14 April 2010); https://doi.org/10.1117/12.851444
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