A system architecture for online data interpretation and reduction in fluorescence microscopy

Thorsten Röder; Matthias Geisbauer; Yang Chen; Alois Knoll; Rainer Uhl

doi:10.1117/12.840223

28 January 2010 A system architecture for online data interpretation and reduction in fluorescence microscopy

Thorsten Röder, Matthias Geisbauer, Yang Chen, Alois Knoll, Rainer Uhl

Proceedings Volume 7538, Image Processing: Machine Vision Applications III; 75380E (2010) https://doi.org/10.1117/12.840223
Event: IS&T/SPIE Electronic Imaging, 2010, San Jose, California, United States

Abstract

In this paper we present a high-throughput sample screening system that enables real-time data analysis and reduction for live cell analysis using fluorescence microscopy. We propose a novel system architecture capable of analyzing a large amount of samples during the experiment and thus greatly minimizing the post-analysis phase that is the common practice today. By utilizing data reduction algorithms, relevant information of the target cells is extracted from the online collected data stream, and then used to adjust the experiment parameters in real-time, allowing the system to dynamically react on changing sample properties and to control the microscope setup accordingly. The proposed system consists of an integrated DSP-FPGA hybrid solution to ensure the required real-time constraints, to execute efficiently the underlying computer vision algorithms and to close the perception-action loop. We demonstrate our approach by addressing the selective imaging of cells with a particular combination of markers. With this novel closed-loop system the amount of superfluous collected data is minimized, while at the same time the information entropy increases.

Citation Download Citation

Thorsten Röder, Matthias Geisbauer, Yang Chen, Alois Knoll, and Rainer Uhl "A system architecture for online data interpretation and reduction in fluorescence microscopy", Proc. SPIE 7538, Image Processing: Machine Vision Applications III, 75380E (28 January 2010); https://doi.org/10.1117/12.840223

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