Intravital multiphoton tomography as a novel tool for non-invasive in vivo analysis of human skin affected with atopic dermatitis

Volker Huck; Christian Gorzelanny; Kai Thomas; Verena Niemeyer; Thomas A. Luger; Karsten König; Stefan W. Schneider

doi:10.1117/12.841973

2 March 2010 Intravital multiphoton tomography as a novel tool for non-invasive in vivo analysis of human skin affected with atopic dermatitis

Volker Huck, Christian Gorzelanny, Kai Thomas, Verena Niemeyer, Thomas A. Luger, Karsten König, Stefan W. Schneider

Author Affiliations +

Proceedings Volume 7548, Photonic Therapeutics and Diagnostics VI; 75480B (2010) https://doi.org/10.1117/12.841973
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Atopic Dermatitis (AD) is an inflammatory disease of human skin. Its pathogenesis is still unknown; however, dysfunctions of the epidermal barrier and the immune response are regarded as key factors for the development of AD. In our study we applied intravital multiphoton tomography (5D-IVT), equipped with a spectral-FLIM module for in-vivo and ex-vivo analysis of human skin affected with AD. In addition to the morphologic skin analysis, FLIM technology gain access to the metabolic status of the epidermal cells referring to the NADH specific fluorescence lifetime. We evaluated a characteristic 5D-IVT skin pattern of AD in comparison to histological sections and detected a correlation with the disease activity measured by SCORAD. FLIM analysis revealed a shift of the mean fluorescence lifetime (tau_m) of NADH, indicating an altered metabolic activity. Within an ex-vivo approach we have investigated cryo-sections of human skin with or without barrier defects. Spectral-FLIM allows the detection of autofluorescent signals that reflect the pathophysiological conditions of the defect skin barrier. In our study the tau_m value was shown to be different between healthy and affected skin. Application of the 5D-IVT allows non-invasive in-vivo imaging of human skin with a penetration depth of 150 μm. We could show that affected skin could be distinguished from healthy skin by morphological criteria, by FLIM and by spectral-FLIM. Further studies will evaluate the application of the 5D-IVT technology as a diagnostic tool and to monitor the therapeutic efficacy.

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Volker Huck, Christian Gorzelanny, Kai Thomas, Verena Niemeyer, Thomas A. Luger, Karsten König, and Stefan W. Schneider "Intravital multiphoton tomography as a novel tool for non-invasive in vivo analysis of human skin affected with atopic dermatitis", Proc. SPIE 7548, Photonic Therapeutics and Diagnostics VI, 75480B (2 March 2010); https://doi.org/10.1117/12.841973

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