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2 March 2010 Endoscopic ICG perfusion imaging for flap transplants: technical development
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Proceedings Volume 7548, Photonic Therapeutics and Diagnostics VI; 75482A (2010)
Event: SPIE BiOS, 2010, San Francisco, California, United States
Objective: Following tumour surgery in the head and neck region, skin flap transplants are usually required to cover the resection area. The purpose of the development was to provide a simple and reliable means to assess whether the transplanted flap is sufficiently perfused. Methods: Fluorescence of intravenously injected Indocyanine green (ICG) was detected with a slightly modified 3-chip CCD camera. Appropriately coated optical filters allow for excitation of ICG with NIR light and detection of NIR ICGfluorescence with the blue channel of the camera. In addition, low intensities of white light can be transmitted to allow for simultaneous display of a remission image in the green and red channels of the camera. Further processing was performed with a LabVIEW program. Results: A satisfactory white light image (red, green and blue display (RGB)) could be calculated from the remission images recorded with the green and red channels of the camera via a look-up table. The look-up table was programmed to provide an optimized blue intensity value for each combination of red and green values. This was generated using a reference image. Implementation of image tracking and intensity measurements in regions of interest (ROIs) in the images is useful to reliably monitor perfusion kinetics of flap and adjacent normal tissue.
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Herbert Stepp, Hilmar Schachenmayr, André Ehrhardt, Werner Göbel, Sven Zhorzel, and Christian Stephan Betz "Endoscopic ICG perfusion imaging for flap transplants: technical development", Proc. SPIE 7548, Photonic Therapeutics and Diagnostics VI, 75482A (2 March 2010);

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