2 March 2010 Using optical coherence tomography to monitor effects of electromechanical reshaping in septal cartilage
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Proceedings Volume 7548, Photonic Therapeutics and Diagnostics VI; 75482J (2010) https://doi.org/10.1117/12.847477
Event: SPIE BiOS, 2010, San Francisco, California, United States
Electromechanical reshaping (EMR) of cartilage is a promising noninvasive technique with potential for broad application in reconstructive surgery. EMR involves applying direct current electrical fields to localized stress regions and initiating a series of oxidation-reduction reactions, thus effecting a shape change. Previous EMR studies have focused on macroscopic structural measurements of the shape change effect or monitoring of electrical current flow. Only limited investigation of structural changes in the tissue at the histologic level have been performed, and not in real time. This study is the first to use optical coherence tomography (OCT) to examine structural changes in cartilage during EMR. Two platinum needle electrodes were inserted into fixed rectangular rabbit nasal septal cartilage specimens. The spectral domain OCT probe was then positioned above the section of cartilage in which the anode needle was inserted. A constant voltage of 6V was applied for 3 minutes, and images were obtained (8 frames/second). OCT was also performed in specimens undergoing dehydration under ambient conditions and during pH changes produced by the addition of HCl, as both processes accompany EMR. The OCT data identified distinct findings among the three conditions, suggesting that EMR causes a much greater degree of reshaping on a molecular level than dehydration or a change in pH alone. OCT provides a means to gauge structural changes in the tissue matrix during EMR. The application of OCT to image the EMR process will add to our understanding of the mechanisms of action involved and potentially facilitate optimization of this process.
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Heather Chen, Lingfeng Yu, Cyrus Manuel, Brian J. F. Wong, "Using optical coherence tomography to monitor effects of electromechanical reshaping in septal cartilage", Proc. SPIE 7548, Photonic Therapeutics and Diagnostics VI, 75482J (2 March 2010); doi: 10.1117/12.847477; https://doi.org/10.1117/12.847477

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