2 March 2010 Structural and biochemical characterization of the rat retina with combined Raman spectroscopy-spectral domain optical coherence tomography (RS-SDOCT)
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Proceedings Volume 7550, Ophthalmic Technologies XX; 75501B (2010) https://doi.org/10.1117/12.843106
Event: SPIE BiOS, 2010, San Francisco, California, United States
Abstract
Raman spectroscopy (RS) and Optical Coherence Tomography (OCT) are powerful tools for optical analysis of tissues with complimentary strengths. OCT excels and visualizing tissue microstructure while RS can relay tissue biochemical composition. Both instruments have significant potential to serve as valuable tools in non-invasive characterization of the rodent models of retinal disease. In this abstract we present the design and application of a common detector combined RS-OCT instrument for evaluating the morphological and biochemical differences in a rat model for oxygen induced retinopathy. Rat pups that have undergone a variable oxygen treatment are compared to rats raised in room air. Images and spectra collected at an age of 26 days postnatal demonstrate differences in both the thickness of the inner and outer nuclear layers, but also in the biochemical composition.
© (2010) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Chetan A. Patil, Chetan A. Patil, Jeroen Kalkman, Jeroen Kalkman, Dirk Faber, Dirk Faber, John S. Penn, John S. Penn, Ton G. van Leeuwen, Ton G. van Leeuwen, Anita Mahadevan-Jansen, Anita Mahadevan-Jansen, } "Structural and biochemical characterization of the rat retina with combined Raman spectroscopy-spectral domain optical coherence tomography (RS-SDOCT)", Proc. SPIE 7550, Ophthalmic Technologies XX, 75501B (2 March 2010); doi: 10.1117/12.843106; https://doi.org/10.1117/12.843106
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