The importance of pulsing illumination parameters in LLLT

D. Barolet

doi:10.1117/12.842901

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17 February 2010 The importance of pulsing illumination parameters in LLLT

D. Barolet

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Proceedings Volume 7552, Mechanisms for Low-Light Therapy V; 755203 (2010) https://doi.org/10.1117/12.842901
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

The influence of emission parameters in Low Level Light Therapy (LLLT) on cellular responses is not yet fully understood. This study assessed the impact of various light delivery modes on collagen production in human primary fibroblast cultured in monolayers after three treatments with red light emitting diode illumination (630 nm, 8 J/cm²). Human type I collagen was measured in cell culture supernatants with procollagen Type I C-Peptide enzyme immunoassay. Results from this study demonstrated that specific μsec pulsing patterns had a more favorable impact on the ability of fibroblasts to produce collagen de novo than comparator conditions of continuous wave, pulsed 50% duty cycle, and millisecond pulsing domain (72 hours post baseline). The cascade of events leading to collagen production by red illumination may be explained by the photodissociation of nitric oxide from cytochrome c oxidase. Short and intermittent light delivery might enhance this cellular strategy.

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D. Barolet "The importance of pulsing illumination parameters in LLLT", Proc. SPIE 7552, Mechanisms for Low-Light Therapy V, 755203 (17 February 2010); https://doi.org/10.1117/12.842901

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