The design of a microfluidic biochip for the rapid, multiplexed detection of foodborne pathogens by surface plasmon resonance imaging

Michael D. Zordan; Meggie M. G. Grafton; Kinam Park; James F. Leary

doi:10.1117/12.842889

25 February 2010 The design of a microfluidic biochip for the rapid, multiplexed detection of foodborne pathogens by surface plasmon resonance imaging

Michael D. Zordan, Meggie M. G. Grafton, Kinam Park, James F. Leary

Author Affiliations +

Proceedings Volume 7553, Frontiers in Pathogen Detection: From Nanosensors to Systems; 755307 (2010) https://doi.org/10.1117/12.842889
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

The rapid detection of foodborne pathogens is increasingly important due to the rising occurrence of contaminated food supplies. We have previously demonstrated the design of a hybrid optical device that has the capability to perform realtime surface plasmon resonance (SPR) and epi-fluorescence imaging. We now present the design of a microfluidic biochip consisting of a two-dimensional array of functionalized gold spots. The spots on the array have been functionalized with capture peptides that specifically bind E. coli O157:H7 or Salmonella enterica. This array is enclosed by a PDMS microfluidic flow cell. A magnetically pre-concentrated sample is injected into the biochip, and whole pathogens will bind to the capture array. The previously constructed optical device is being used to detect the presence and identity of captured pathogens using SPR imaging. This detection occurs in a label-free manner, and does not require the culture of bacterial samples. Molecular imaging can also be performed using the epi-fluorescence capabilities of the device to determine pathogen state, or to validate the identity of the captured pathogens using fluorescently labeled antibodies. We demonstrate the real-time screening of a sample for the presence of E. coli O157:H7 and Salmonella enterica. Additionally the mechanical properties of the microfluidic flow cell will be assessed. The effect of these properties on pathogen capture will be examined.

Citation Download Citation

Michael D. Zordan, Meggie M. G. Grafton, Kinam Park, and James F. Leary "The design of a microfluidic biochip for the rapid, multiplexed detection of foodborne pathogens by surface plasmon resonance imaging", Proc. SPIE 7553, Frontiers in Pathogen Detection: From Nanosensors to Systems, 755307 (25 February 2010); https://doi.org/10.1117/12.842889

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