17 February 2010 Multiplex detection of disease marker proteins with arrayed imaging reflectometry
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Abstract
Arrayed Imaging Reflectometry, or "AIR", is a new label-free optical technique for detecting proteins. AIR relies on binding-induced changes in the response of an antireflective coating on the surface of a silicon chip. Thus far, we have demonstrated the use of AIR for the detection of pathogenic E. coli, and for multiplex detection of a broad range of proteins in human serum. Creation of the near-perfect antireflective coating on the surface of silicon requires careful control over preparation of the chip surface prior to probe molecule immobilization. We present methods for highly reproducible, solution-phase silanization and glutaraldehyde functionalization of silicon chips carrying a layer of thermal oxide. Following functionalization with antibodies and passivation of remaining reactive groups, these surfaces provide exceptional performance in the AIR assay.
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Amrita Yadav, Rashmi Sriram, Benjamin L. Miller, "Multiplex detection of disease marker proteins with arrayed imaging reflectometry", Proc. SPIE 7553, Frontiers in Pathogen Detection: From Nanosensors to Systems, 75530J (17 February 2010); doi: 10.1117/12.847363; https://doi.org/10.1117/12.847363
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