17 February 2010 Multiplex detection of disease marker proteins with arrayed imaging reflectometry
Author Affiliations +
Arrayed Imaging Reflectometry, or "AIR", is a new label-free optical technique for detecting proteins. AIR relies on binding-induced changes in the response of an antireflective coating on the surface of a silicon chip. Thus far, we have demonstrated the use of AIR for the detection of pathogenic E. coli, and for multiplex detection of a broad range of proteins in human serum. Creation of the near-perfect antireflective coating on the surface of silicon requires careful control over preparation of the chip surface prior to probe molecule immobilization. We present methods for highly reproducible, solution-phase silanization and glutaraldehyde functionalization of silicon chips carrying a layer of thermal oxide. Following functionalization with antibodies and passivation of remaining reactive groups, these surfaces provide exceptional performance in the AIR assay.
© (2010) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Amrita Yadav, Amrita Yadav, Rashmi Sriram, Rashmi Sriram, Benjamin L. Miller, Benjamin L. Miller, } "Multiplex detection of disease marker proteins with arrayed imaging reflectometry", Proc. SPIE 7553, Frontiers in Pathogen Detection: From Nanosensors to Systems, 75530J (17 February 2010); doi: 10.1117/12.847363; https://doi.org/10.1117/12.847363

Back to Top